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Lipase Suicide Inhibitors


Custom synthesis of suicide inhibitors with varying chain lengths and/or leaving groups

In addition to the portfolio of novel lipases, EUCODIS Bioscience now introduces a range of suicide inhibitor substrates for lipases. The inhibitor substrates are designed for active site titration experiments and, e.g. can be used for the quantitative determination of active lipases and esterases in complex protein mixtures. They are ideally suited for applications in development and quality control of hydrolytic enzymes like lipases and esterases, and are used for these applications in house.

These phosphonate based molecules carry either chromogenic (p-nitrophenyl) or fluorogenic (4-methyl-umbelliferyl) groups for sensitive detection of hydrolytic activity of lipases and esterases. The release of p-nitrophenol can be detected photometrically at 405 nm, whereas the more sensitive fluorescence substrate releases 4-methylumbelliferone which shows strong fluorescence with increasing intensity at higher pH (pH 8: λex 363 nm; λem 465 nm).

Please contact the EUCODIS Bioscience product support for additional information on the suicide inhibitors and for support on your specific application using our new lipase/esterase suicide inhibitor substrates.

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Example of an active site titration

 

Recommended usage

All suicide inhibitor substrates are both soluble in DMF and DMSO.
Active site titrations should be performed in a pH range from 7-8
considering that higher pH values lead to appreciable spontaneous hydrolysis.

4-MU (pH 8) λex: 363 nm λem: 465 nm
4-pNP (pH 8) ε 405 nm= 14280 mol*l-1*cm-1

 

Overview chromogenic suicide inhibitor substrates

ESIC01:
Methyl 4-nitrophenyl hexylphosphonate 10mg
FW: 301.28
Chromogenic standard substrate for the determination of active sites
in lipases.

ESIC02:
Butyl 4-nitrophenyl hexylphosphonate 10mg
FW: 343.36
Chromogenic substrate for the determination of active sites in lipases
preferring long chain leaving groups.

ESIC03:
Octyl 4-nitrophenyl hexylphosphonate 10mg
FW: 399.46
Chromogenic substrate for the determination of active sites in lipases
preferring hydrophobic leaving groups.

 

Overview fluorigenic suicide inhibitor substrates

 

ESIF01:
Methyl 4-methyl-umbelliferyl hexylphosphonate 2mg
FW: 338.34
Fluorigenic standard substrate for the determination of active sites in lipases.