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Tubulysins

Written by Susanne Bach. Posted in products

Natural tubulysins and analogues

Our Partner Tube Pharmaceuticals has developed several tubulysins for use in conjugate therapeutics which are produced by fermentation at EUCODIS Bioscience. The potency of Tube’s tubulysin toxins as a conjugate payload allows to target resistant cancers.

The toxins are available in milligram to  gram quantities. Please contact the EUCODIS Bioscience product support for a price offer, additional information on tubulysins and for support on your specific application using our new compounds.

Available toxins are:

  • Tubulysin A
  • Tubulysin B
  • Other natural or synthetic analogues on request

 

Tubulysin A: R1 = CH2CH(CH3)2
Tubulysin B: R1 = CH2CH2CH3

>> Download datasheet

Like auristatins and maytansine, which are used as payloads in antibody-drug conjugates (ADCs), tubulysins are  microtubule-disrupting agents. The recently discovered natural product class of the tubulysins shows a very high cytotoxic activity in in-vitro and in-vivo tumor models, especially in tumor cells resistant against other microtubule destabilizing drugs.

Many representatives of these natural products are several orders of magnitude more potent than other marketed chemotherapeutics such as vincristine, taxanes and others. Based on the structure-activity relationship  of the tubulysins, this class allows many conjugation and targeting strategies: They are well suited for any kind of conjugation to polymers or biomolecules such as e.g. antibodies or proteins.

Tubulysins are a naturally occurring cytotoxic class of peptide compounds which destabilize microtubules and prevent cell growth and division1. Their known biologic activities can be summarized as follows:

  • Tubulysins bind to the vinca domain of tubulin with higher affinity than vinblastine, leading to tubulin depolymerisation, G2/M accumulation and apoptosis2.
  • Tubulysins are approximately 100- to 1000-fold more cytotoxic than traditional chemotherapeutics (e.g. taxol, vinblastine) with EC50 in the nanomolar to sub-nanomolar range.
  • Studies indicate that tubulysins are highly active in multidrug resistant cell lines that either overexpress P-glycoprotein pumps or have tubulin mutations3.
  • Tubulysin A is not a substrate for cytochrome P450 enzymes, making unfavourable drug-drug interactions less likely3.


Crystals of tubulysin A

Picture kindly provided by Prof. G. Hoefle, GBF Braunschweig, Germany

References:
1. J. Antibiotics, 53: 879 (2000); G. Höfle et al.
2. Chembiochem 7:678 (2006); Khalil et al.
3. Biochem. J. 396: 235-242 (2006); A. Dömling et al.

Further reading:
1. Mol. Cancer Ther., 15(5) (2016); P.J. Burke et al.
2. Molec. Div. 141 (2005); A. Dömling, W.Richter
3. Ang. Chem. Intl. Ed. Engl. 4888 (2004); G. Höfle et al.



 

 

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Peroxidases

EUCODIS has selected over 20 novel oxidative enzymes for industrial applications. The enzymes enable biocatalytic oxidation reactions beyond known dehydrogenases, P450 enzymes, reductases:

Benefits:

  • highest activity, specificity and selectivity
  • mild reactions conditions protecting your substrate
  • minimize side reactions, reducing waste and process energy
  • hydrogen peroxide as co-substrate, eliminating the need of cofactor recycling systems.

For more information please download our product flyer.

To see our most recent achievments in peroxidase development please download our poster from OxiZymes Vienna 2014.

This email address is being protected from spambots. You need JavaScript enabled to view it. will be glad to answer your questions regarding our new peroxidases.

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Lipases

Written by Susanne Bach. Posted in products

EUCODIS Bioscience offers a collection of more than 40 unique lipases of microbial origin, carefully selected and engineered for applications in the chemical, food & feed industry and pharmaceutical.

Twenty-five enzymes are available as a screening Kit enabling rapid selection for your application. Alternatively, EUCODIS also offers a screening service on request or development of lipase formulations tailored to your application. In addition, we also can provide Immobilized lipases for easy recovery and repeated use or continuous bioreactors available on request.

Superior activity in multiple applications

  • Biocatalytic stereospecific resolution of chiral alcohols and acids for API synthesis/fine chemistry
  • Generation of difficult to synthesize, sensitive esters
  • Production of nutritionally valuable fats and oils
  • Selective and efficient hydrolysis/Esterification of C4-C22 fatty acids
  • Emollient ester synthesis for cosmetics
  • Bioplastic precursor modification refinement

Our novel collection of lipases can be purchased as a screening kit or individually from mg to kg amounts. Upon requests, bulk quantities or higher purity lipase preparations can be supplied – tailored to match the application of our customers. All lipases are expressed in media with animal-free components, please inquire for lipases expressed in GRAS organisms.

For more information please download our product flyer.

If you want to receive a quote please This email address is being protected from spambots. You need JavaScript enabled to view it. .

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β-Lactamase

Written by Christian Siehs. Posted in products

EUCODIS manufactures and delivers pure, high-quality, high-performing β-lactamases. LacBuster™ has been optimized for sterility testing and environmental monitoring in the manufacture and dosage formulation of beta-lactam antibiotics and for specific diagnostic purposes.

LacBuster™-S is a novel β-lactamase formulation with a broad substrate range against β-lactam antibiotics including carbapenems, cephalosporins up to 5th generation and penicillins. LacBuster™ addresses the needs of leading antibiotics manufacturers and laboratories interested in sterility testing of biological specimens and shows an activity significantly higher than that of other products currently in the market.

LacBuster™-L is a novel sterile ready-to-use solution. Due to its high performance LacBusterTM-L is especially well suited for the direct inoculation method and membrane filtration tests according to US Pharmacopeia (USP <71>) and EP. It can be directly implemented into existing standard operating procedures. Please contact our application specialists regarding usage.

Penase is a Penicillinase with a specific substrate range solely against all relevant penicillins. Penase is available as a freeze-dried product.

For more information please download our product flyer.

If you want to receive a quote or learn more about LacBuster™ product specifications please This email address is being protected from spambots. You need JavaScript enabled to view it. .

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β-Lactamase media plates

Posted in products

EUCODIS manufactures and delivers pure, high-quality, high-performing products. LacBuster™ has been optimized for sterility testing and environmental monitoring in the manufacture and dosage formulation of beta-lactam antibiotics.

LacBuster™ Agar Plates for environmental monitoring

Eucodis provided a general purpose growth medium supplemented with LacBuster™ to effectively neutralise beta-lactam antibiotics. The media plates are suitable for the cultivation of a wide variety of microorganisms in environmental monitoring within the pharmaceutical industry.

Available as contact and settle plates for high performance monitoring of your isolators and clean rooms during beta lactam manufacture.

Why use LacBuster™ media plates?

  • High enzymatic activity of 1.000 IU Cephalosporinase and 10.000 IU Penase per litre medium
  • Optimized formulation: Supplemented with neutralizers for all commonly used disinfectants
  • H2O2-impermeable triple-sleeve packaging
  • Gamma-irradiated (20 – 35 kGy)
  • Long shelf life: 6 months
  • Convenient storage at room temperature

For more information please download our product flyer.

If you want to receive a quote or learn more about LacBuster™ Agar Plates product specifications please This email address is being protected from spambots. You need JavaScript enabled to view it. .