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In Vivo Recombination Technology

EUCODIS Bioscience exploits the industrial potential of recombination in living cells (in vivo recombination) through the precise control of the DNA Mismatch Repair System. This tool represents a versatile and powerful technology for the development of enzymes optimized for specific industrial applications.

Our technology is based on the recombination of genes - Nature's "tool" for the creation of genetic diversity. Recombination has been perfected in hundreds of millions of years of evolution. This concept is different from mutation, Nature's second source of diversity: Mutations mean purely random changes in a DNA sequence. By contrast, recombination creates diversity by combining functional genetic "building blocks" in a novel way.

In a natural environment, however, only similar (homologue) DNA sequences can be recombined. The DNA Mismatch Repair System prevents recombination between diverged DNA sequences, such as sequences from different species. Our technology allows to selectively switch off this control mechanism in organisms such as yeast or bacteria, enabling the recombination of variants of a gene with only 65 percent homology.

 

in vivo recombination

The key advantage of our technology is its efficacy. Already after the first round of recombination, our technology generates high-quality libraries of mosaic genes or genomes, consisting only of true recombinants. A high proportion of novel enzymes created with this technology are functional, because crossovers only occur at sequence-defined domains.

Moreover, no frame-shifts, stop codons, insertions or deletions are generated, leaving the integrity of the resultant 3D-structures undisturbed.

The resulting recombinants are then screened to identify genes encoding enzymes with novel or improved functions.

Our proprietary technology of in vivo recombination is patent-protected.

For detailed information on scientific literature and current publications related to our technology of in vivo recombination, please contact Dr. Jan Modregger, Head of Research and Development.